Stem Cells are undifferentiated cells that can turn into specific cells, as the body needs them. They have two characteristics:
- They can replicate themselves, or;
- They can differentiate into many other cell types, including bone, cartilage, muscle, nerves and connective tissue.
micro-vesicles (30-150nm) that are involved in cell communication. This is a new frontier in regenerative medicine. These exosomes have been recently discovered to be involved in direct cell signaling. Exosomes are isolated from donated human mesenchymal stem cells (MSCs) and purified using our proprietary processing. The unique benefit to these exosomes is that they are stem cell-derived and bring a wide array of growth factors to stimulate and modulate many processes within the healing cascade. Exosomes are not cells but are micro-vesicles, which are about 1/1,000th the size of a cell and contain no nucleus or DNA. They can be regarded as the purest form of cellular therapy available, because their function is to direct tissue and wound healing by activating the patient’s own regenerative cell response. This is one of the latest generations of naturally bioactive stem cell-based products for patient treatment. Stem cells and stem cell therapy work by paracrine signaling (local cell-to-cell communication to induce changes in nearby cells) to create the appropriate healing environment to restore tissue. The exosomes are those paracrine signals isolated from stem cells that can be delivered without the need for delivering stem cells. As the technology and discovery of new methods of treating patients has evolved, the science shows that the optimal way to provide true “stem cell therapy” is to directly provide the signals as its own therapy and avoid potential issues with delivering and keeping transplanted cells alive. Stem cells by nature work to heal, repair, and restore the body. But, in some cases where there is a chronic abnormality, these cells fall into an inflammatory state, work in an unregulated capacity and inhibit healing. The 1000+growth factor proteins found to naturally occur in healthy tissue have shown to help modulate the inflammatory healing process. This is accomplished by activating and recruiting the patients “own cells” to the area, revascularizing the area and reducing the inflammatory response to allow natural healing.
Yes. VitaCell Biologics is an FDA registered tissue bank, specializing in the processing, storing and distribution of Umbilical Cord Tissue. We are an inspected and established HCT/P facility operating under the guidelines per the 21 CFR 1271.10 of the PHS Act, Section 361. We work closely with clinicians and practitioners strategically placed across the U.S. with the focus of enhancing lives through natural biologics, skincare and supplement products.
Click on this link to see our FDA Registration:
Stem cells are progenitor cells which are capable of regeneration and differentiation into a wide range of specialized cell types. Once injected, stem cells follow inflammatory signals from damaged tissues and have multiple ways of repairing these damaged areas.
The mesenchymal stem cells (MSCs), from the Umbilical Cord, are considered to be multipotent and can transform into different cell types.
They act via anti-inflammatory activity, immune modulating capacity, and the ability to stimulate regeneration. We go through a very high throughput screening process to find cells that we know have the best anti-inflammatory activity, the best immune modulating capacity, and the best ability to stimulate regeneration.
At VitaCell Biologics, we utilize adult stem cells derived from Human Umbilical Cord Tissue. This has the highest source of mesenchymal stem cells (MSCs). These cells ideally assist in the treating of systemic autoimmune and inflammatory conditions. They also play a significant role in cellular signaling to repair and regenerate injured tissue.
The umbilical cord is processed in the hospital according to the rules and regulation of the American Association of Tissue Banks (AATB). Approximately 4 weeks prior to a scheduled caesarean section, the mothers OB/GYN asks her if she would like to keep and store her unborn child’s umbilical cord for future use. If the mother declines, then she is asked if she would like to donate the umbilical cord. If she agrees, she undergoes a review of her medical history, social history, and a blood test. If she is deemed an acceptable donor according to prevailing rules of the AATB, then at the time of her caesarean section, an experienced technician will clamp the umbilical cord, take it to a clean room and place it into a sterile collection bag. The bag of umbilical cord is then delivered to the lab for processing. Once at the lab, a sample of the umbilical cord tissue is sent to a 3rd
party independent FDA registered lab for testing according to United States Pharmacopeia rule 71 (U.S.P. 71), which is a test for known communicable diseases. While that test is taking place, the stem cells are then processed and red blood cell products are removed. A sample of the finished stem cell product is then sent to a different 3rd party independent FDA registered lab for sterility testing. Only after both lab reports come back as “clean” and have passed the regulatory requirements, are the processed umbilical cord stem cells available for distribution.
Yes, as long as you store them at -80 C. We have commercially available -80 C freezers that will allow you to buy in bulk and have the cells available for immediate use. The freezer option will also save you shipping costs associated with multiple monthly shipments.
No, DNA testing is not necessary because the stem cells and mononucleated cells do NOT penetrate the nucleus of the recipients’ cells and thus do not pass on DNA. The ability of a stem cell to pass along DNA matter is a property of a stem cell when it is in the embryonic stage and is no longer possible after the 10th week of gestation. Umbilical cord stem cells are harvested between weeks 36 and 40.
The International Society for Cellular Therapy (ISCT) Says That the Presence of CD90, CD105, and CD73 are Necessary for the Identification of Stem Cells. Why do you Only Test for the Presence of CD90?
The definition by the ISCT is for pure stem cells and not a heterogenous mix of stem cells and mononucleated cells that are used for umbilical cord stem cell therapies. It is also noted in literature that the presence of one or all the CD’s is consistent with the presence of stem cells is an indicator of stem cells. The reason we chose to test for CD90 is because it is the most clinically relevant for our purposes. CD90 is implicated in axonal growth, T-cell activation, cell adhesion, cell migration, and cell extravasation while CD105 is implicated in hematopoietic stem cell (HSC) function and CD73 is implicated in tumor suppression.
Research has shown that the vast majority of the function of stem cells is for Medicinal Signaling and NOT differentiation. Stem cells produce long-term effects by responding to signals from injured tissue. The stem cells work by inhibiting the inflammatory components that cause pain and damage to tissue and the mononucleated cells secrete growth factors, proteins, and cytokines that stimulate our own native tissue to repair and regenerate itself.
Yes, allogeneic (taken from another person of the same species) cells are safe to put into your body. When the umbilical cord is processed, all the red blood cell components that could cause a negative reaction are removed. Also, the umbilical cord cells are naïve/immature and do NOT react the way a mature adult cell would act.
“Mesenchymal stem cells produce huge quantities of bio-molecules, some of which are immunosuppressive; MSC’s put up a curtain of molecules around themselves that allows donor (allogenic) MSC’s to be transplanted into a recipient, free from an immune response. (Immune privileged)”
Arnold Caplan, PhD. Case Western Reserve University. Experimental and Molecular Medicine (2013) 45
Mesenchymal stem cells: environmentally responsive therapeutics for regenerative medicine
Yes, age definitely matters. In Vitro research by the International Journal of Molecular Sciences has shown that while young, vibrant umbilical cord stem cells can duplicate themselves every 28 hours for up to 65 generations or more, 50+ year old bone marrow aspirate and adipose derived stem cells duplicate at a much slower rate of 3 to 5 days for an average of only 11 to 13 generations. Also of note is that older cells undergo senescence (aging) at a much faster rate than young, vibrant umbilical cord cells and older senescent cells produce less quantities and less effective growth factors, proteins, and cytokines.
Int. J. Mol. Sci. 2013, 14, 17986-18001; doi:10.3390/ijms140917986. International Journal of Molecular Sciences ISSN 1422-0067 www.mdpi.com/journal/ijms
Yes, you may mix/dilute the cells with PRP, which is isotonic and contains growth factors so it can increase the anti-inflammatory component.
You may mix/dilute the cells with LACTATED RINGERS, normal saline (0.9%), or Hank’s Balanced Salt Solution. LACTATED RINGERS is readily available and causes minimal hyperosmolality. With normal saline, up to 10% to 15% of the cells could lyse. The lysed cells will still exert the function of the growth factors, by only the half-life of that growth factor, similar to amniotic fluid. (Cell death from the use of saline and thawing is considered when the cells are initially concentrated so that you will have the labeled number of cells after both processes)
Yes, 60%-70% is very good viability after thawing. In a clinical setting, (placing the vial in the palm of your hand for 3 to 4 minutes) viability of 50% to 70% is well documented as a good viability rate.
VitaCell Biologics viability rate, after thawing, is in the high 90% range.
*It should be noted that in a laboratory setting, cell viability, with all the technical and reagent advantages, can yield viability counts of over 90%.
- Less invasive when compared to bone marrow / adipose aspiration
- Less pain for the patient and much shorter procedure time
- Umbilical Cord Wharton’s Jelly lack MHC-II and therefore are hypo-immunogenic
- Fibroblast colony forming units are significantly higher in Umbilical Cord Wharton’s Jelly
- Faster population rate
- Have strong anti-inflammatory effect, reducing the expression of inflammatory cytokines
- High concentration of peptides, hyaluronic acid and growth factors
- Increases the likelihood for better patient outcomes
- pain management
- sports injuries
- spinal conditions
- wound care
- male enhancement
- vaginal rejuvenation
- hair restoration
- Knee joint injury, tears of the meniscus
- Hip and Shoulder pain
- Chronic partial rotator cuff tears
- Persistent partial tendon tears (tennis elbow, golfer’s elbow)
- Plantar fasciitis (bone spurs)
- Achilles tendonitis
- Quadriceps and Patellar tendon tears
- Muscular tears
- Degenerative arthritis of the Knee, Hip, Shoulder or Ankle
- Intervertebral disc and spinal facet joints
- Sacroiliac joint pain
- Greater trochanteric bursitis
- Chronic neck pain, chronic back pain